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. 2012 Dec 27;7(12):e53119. doi: 10.1371/journal.pone.0053119

Figure 1. Confluence- and suspension-cultured mammary epithelial cells demonstrate increased psoriasin and CD24 expression.

Figure 1

MCF10A cells were cultured in confluence for 5 and 10 days or in suspension for 3 days. A Psoriasin expression, analyzed by Western blotting, was induced in MCF10A cells cultured in confluence and suspension, compared with exponentially growing cells. Equal loading was confirmed by GAPDH. The figure illustrates a representative example (n = 3). B In confluence- and suspension-cultured cells, psoriasin and CD24 expression were increased, whereas CD44 expression was decreased compared with exponentially growing cells. The expression level of CD24 and CD44 was measured using flow cytometry and the expression level of psoriasin was quantified from Western blots. The data are presented as the mean ± SD of relative expression (n = 3). The p-values (*<0.05, **<0.01, ***<0.001) were calculated using the Student's t-test.