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. 2012 Dec 27;7(12):e53119. doi: 10.1371/journal.pone.0053119

Figure 4. Endogenous psoriasin causes increased CD24 expression.

Figure 4

MCF10A cells were transfected with a psoriasin-targeting shRNA (Pso-shRNA) or siRNA (Pso-siRNA), and their corresponding controls,(C-shRNA and C-siRNA). Transfected MCF10A cells were cultured in confluence for 10 days or in suspension for 3 days. The expression level of psoriasin was detected by Western blot. CD24 and CD44 expression were measured using flow cytometry. A No induction of psoriasin expression was observed in Pso-shRNA, compared with C-shRNA, in confluence or suspension. B Pso-shRNA showed a decrease in the expression of CD24 and an increase in the expression of CD44, compared with C-shRNA, during confluence and suspension. C The expression of psoriasin in Pso-siRNA was dramatically downregulated, compared with C-siRNA. D Pso-siRNA in suspension culture showed a reduced CD24 expression, compared with C-siRNA. Equal loading was confirmed by GAPDH. The figures illustrate representative examples (n = 3). The data are presented as the mean ± SD of relative expression (n = 3). The p-values (*<0.05) were calculated using the Student's t-test.