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. 2012 Dec 27;7(12):e51972. doi: 10.1371/journal.pone.0051972

Figure 3. S961 stimulates IR and Akt phosphorylation in CHO-hIR cells.

Figure 3

Cells were stimulated with increasing concentrations of HI or S961. A-E, IR tyrosine phosphorylation. The 6 tyrosine phosphorylation sites which were examined were Y972 in the juxtamembrane domain, Y1158 and Y1162/1163 in the tyrosine kinase domain, and Y1328 and Y1334 in the C-terminal tail end of the IR. F, Akt phosphorylation. Phosphorylation of Ser437 is known to be required for Akt activation. Panels A-E: the increased tyrosine phosphorylation of the IR was significant (compared to 0.0001 nM, 0.001 nM and 0.01 nM S961, P<0.05*, P<0.01**, P<0.001***, two-tailed t-test). Panel F: the increased serine phosphorylation of Akt was significant (compared to 0.0001 nM, 0.001 nM and 0.01 nM S961, P<0.01**, two-tailed t-test). Data points represent average of three independent experiments, each comprising triplicate determinations. Error bars show one standard deviation.