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. 2012 Dec 27;7(12):e53498. doi: 10.1371/journal.pone.0053498

Figure 1. FACS and immunofluorescence analysis show Slug restricted expression by basal-like cells.

Figure 1

A. Using FACS, primary mouse mammary cells were sorted into CD24 Neg (stromal cells: S), CD24 High/CD49f Med (luminal-like cells: Lu) and CD24 Med/CD49 High (basal-like cells: Ba). Results of two experiments are averaged to show expression levels of CK14, CK18, Slug and Snail as quantified by RT-qPCR and normalized to GAPDH reference gene. B. Double immunofluorescence study of mouse mammary epithelial cell line CommaDβ cells show co-expression of Slug and CD49f by a defined sub-population (arrows). In contrast, Slug and CK8 are found mutually exclusive from each other (arrowheads). C. Double immunofluorescence study was quantified for CK5 (white bar), CK8 (white bar) and co-expressed Slug (black bar inside white bar). No CK8+ cell were found to coexpress Slug. CommaDβ were also sorted based on Sca-1 expression as previously described (Deugnier et al., 2006). Sca-1-high basal-like (Ba) and Sca-1-low luminal-like (Lu) cell populations were analyzed for CK14, CK18, Slug, Pcad and Snail. Expression levels obtained by RT-qPCR were normalized to GAPDH reference gene. Data are shown as the mean (+sd) of 3 independent immunobead sorting experiments.