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. 2012 Dec 27;7(12):e52162. doi: 10.1371/journal.pone.0052162

Figure 4. Effects of Harmine on endothelial cell apoptosis.

Figure 4

, G2/M arrest and proliferation. (A) The proportion of apoptotic cells was increased by harmine. Harmine induced cleavage of PARP and suppressed the expression of survivin and Bcl-2. The amount of cleaved PARP and the expression levels of survivin and Bcl-2 in harmine-treated HUVECs were detected by western blot analysis with specific antibodies. (B) Cell cycle arrest of HUVECs by various concentrations of harmine. Cells were treated with various concentrations of harmine for 48 hours, and then the cell cycle was analyzed by flow cytometry. (C) Growth inhibition of HUVECs by harmine, as determined by an MTS assay. All of the experiments were performed followed by addition of harmine for 48 hours. *, P<0.05; **, P<0.01; ***, P<0.001.