Figure 1. Exogenous Nesfatin-1 slightly up-regulates PS.

Effects on PS of acute icv administration of a high dose of Nesfatin-1 (vs. saline, n = 10). Note the modest effect on cumulative PS amounts. The quantitative analysis is organized into 2 h blocks during the first 8 h following administration of Nesfatin-1 (open circles) or saline (filled circles). Cumulative PS quantities (left panel), number of PS bouts (second panel) and a histogram comparing the duration of PS bouts calculated over the 8 h time period post-treatment (vs. control) (right panel) are shown. Values are presented as mean ± SEM and significance values indicated for individual points vs. control with * p<0.05; ** p<0.01, *** p<0.001. For the first two columns, a two factor (time×treatment) within subject ANOVA followed by post hoc Bonferonni test was utilized, while in the third column statistics were performed using a two tailed paired t-test.