Table 1. Correlation between sleep quantities (SWS and PS) and consequent c-Fos expression in Nesfatin-1-immunoreactive cell bodies within THA for the 4 experimental groups considered, i.e. Control, PS deprived, Bad-Sleeper and Good-Sleeper rats.

		Control	PS deprivation	PS recovery
				Bad-Sleepers	Good-Sleepers
Sleep	% PS	13.5±0.9	4.9±1.2**	14.3±1.4a	30.9±1.1***a,c
	% SWS	50.5±0.2	55±4.1	60.3±4.4	56.5±2.3
THA	Fos+	225,7±28,6	635±40,7***	585,3±74,7**	737,5±84,6***
	Fos/Nesf-1+	11,2±2,9	4,4±2,6	48,3±27	194,5±22,3***a,c
ZI-Subl	Fos+	55,2±8,8	110,2±14,5	147,5±23,2*	176,3±24,2**
	Fos/Nesf-1+	3,6±0,6	0,4±0,4	14±7	61,3±12,5***a,c
PeF-LH	Fos+	80,7±13,0	216,6±12,7***	199±24,9**	261,3±27,5***
	Fos/Nesf-1+	5,6±1,5	1,8±1,2	22,8±12,9	101,25±11,1***a,c
L-THA	Fos+	135.9±19.9	326.8±24.7**	346.5±43.8**	437.5±48.8***
	Fos/Nesf-1+	9.2±2.1	2.2±1.6	36.8±19,9	162.5±19.1***a,c

For each experimental condition, the percentage of time spent in SWS and PS scored per 5-sec epoch during the last 2 h before sacrifice is reported (mean ± SEM, n = 5 rats for Control and PS deprivation, n = 4 rats for both PS recovery samples). Then, the number (mean ± SEM) of Fos+, Nesf-1+ and Fos+/Nesf-1+ double-labeled neurons was estimated, bilaterally, for each discrete THA nucleus known to contain Nesf-1+ neurons (see Methods for detailed quantification). Here are reported quantitative data for single-labeled Fos+ and double-labeled Fos+/Nesf-1+ cell bodies when considering either the whole THA and separately, the lateral half of THA (formed by the ZI-Subl and PeF-LHA areas) which contains the majority of the THA Nesf-1+ neurons. Significance values are:

^*p<0.05, **p<0.01, ***p<0.001 vs. control; ^a p<0.001 vs. privation and ^b p<0.05, ^c p<0.001 between bad sleepers and good sleepers, two-way ANOVA followed by a Bonferonni post hoc test.