TABLE 1.
Oligonucleotides used in this study
| Oligonucleotide | Sequencea |
|---|---|
| O1 | 5′-CGCGCGCGCCCCGGGGTTTGGCTGGCCGAACATTTGGTCAAA-3′ |
| O2 | 5′-ACTGGCGTGCCGCGGACGTACCTTCAGCGTTGCCAGACCAATCAG-3′ |
| O3 | 5′-CGCGCGTCTGCAGCTCCGCGGACGCCAGTGTCAGAAAAACAGCTGGC-3′ |
| O4 | 5′-GCGCGCAAGCTTTTACTGTAGCGCCTCGAGGATATGGTATGC-3′ |
| O5 | 5′-TCGAGGCGCTACAGCACCATCACCATCACCATTAATAAT-3′ |
| O6 | 5′-CTAGATTATTAATGGTGATGGTGATGGTGCTGTAGCGCC-3′ |
| O7 | 5′-GTGAAAACCTGTACTTCCAAGGGC-3′ |
| O8 | 5′-CCTTGGAAGTACAGGTTTTCACGC-3′ |
a
Restriction sites introduced for cloning of mraY and P99 β-lactamase genes as detailed in the text are indicated in bold face.