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. 2012 Dec 27;7(12):e53040. doi: 10.1371/journal.pone.0053040

Figure 1. Decreased LC3-II levels in the cerebellum of essential tremor (ET) cases and controls.

Figure 1

LC3-II and β-actin levels in cerebellar homogenates were determined by Western blot in 10 ET cases and 11 controls. Two LC3 antibodies were used, LC3 antibody, Novus Biologicals 1384 (top panel), and LC3-II specific antibody Novus Biologicals 19167 (third panel) and the representative bands were shown (A). LC3-II levels (mean ± SD) were significantly lower in ET cases vs. controls (B). In a linear regression model, ET disease duration inversely correlated with LC3-II level (C).When dividing our sample into three categories, ET cases with the longest disease duration had the most diminished LC3-II levels, followed by ET cases with shorter duration disease and then controls (D). ET cases displayed lower levels of calbindin than age-matched controls, consistent with PC cell loss (A, E). We also determined the LC3-II and β-actin levels in the occipital cortex in 7 ET cases and 9 controls and the representative blots were shown (F). ET cases and controls exhibited similar LC3-II levels in the occipital cortex (mean ± SD) (G).