Skip to main content
. 2012 Dec 27;7(12):e53040. doi: 10.1371/journal.pone.0053040

Figure 3. Mitochondrial accumulation and beclin-1 deficiency in ET cerebellum.

Figure 3

Levels of mitochondrial membrane protein, TIM23 and TOMM20, in cerebellar cortex homogenates in 10 ET cases and 11 controls were determined by Western blot and the representative bands were shown (A). TIM23 and TOMM20 were normalized against β-actin to determine the protein levels. TIM23 and TOMM20 protein levels were significantly higher in the cerebellum of ET cases than controls (B, C). In contrast, TIM23 and TOMM20 protein levels were similar in the occipital cortex of 7 ET cases and 9 controls (D–F). S6K, pS6K, and beclin-1 levels in cerebellar cortex homogenates were determined by Western blot (G). pS6K levels were highly variable (G). pS6K and S6K ratio did not differ between ET cases and controls (H). Beclin-1 level was significantly lower in ET cases than controls (I).