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. 2012 Dec 27;7(12):e53029. doi: 10.1371/journal.pone.0053029

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© 2012 Hardoim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PCR-DGGE 16S rRNA gene fingerprints of S. spinosulus, I. variabilis and seawater DNA samples generated with “total-community” bacterial primers (A) and specific primer systems for Actinobacteria (C) and Alphaproteobacteria (E). The arrows indicate bands that were excised from DG-gels and sequenced. Corresponding ordination biplots of PCR-DGGE fingerprints and qualitative environmental variables are shown in panels B, D, F. Symbols: ▴ S. spinosulus, ▪ I. variabilis and • Seawater. Labels displayed on the diagram axes refer to the percentage variations of PCR-DGGE ribotypes - environment correlation accounted for the respective axis. The “star” symbols represent the centroid positions of the environmental variables in the diagram. Variables that significantly (p<0.05) influence the bacterial community composition are indicated by an asterisk.