Figure 4. Purification of AdhE-D2-iLOV (P3) is easy to follow due to the distinctive yellow-green colour of the iLOV tagged protein, (A).

Purified AdhE-D2 lacking the iLOV domain is colorless, (B). Purified P3 shows fluorescence under ultraviolet light, (C). Purified AdhE-D2 lacking the iLOV domain viewed under uv light (D). The iLOV domain can be readily cleaved from purified proteins using C3 protease, (E). Lane M: Markers, Lane 1: AdhE-D2-iLOV, indicated by arrow, Lane 2: AdhE-D2, iLOV and 3C protease indicated by arrows. The iLOV-His₈ tag has been cleaved off, and the size of AdhE-D2 has been reduced by approximately 16 kDa.