Figure 3. Western blot analysis of cell lysates for phosphorylated AMP-activated protein kinase (pAMPK) and the endosomal adaptor protein APPL-1 (adaptor protein with phosphotyrosine binding, pleckstrin homology domains and leucine zipper motif).

Immunoblotting demonstrated that SH-SY5Y cells transfected with Sw-APP mutant (Sw-APP) had increased pAMPK than SH-SY5Y cells transfected with wt-APP (APP) which had increased pAMPK than control cells transfected with empty-vector (vector). Expression of APPL1 was indifferent among the three groups of cells. Experiments were repeated 3 times with consistent results.