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. 2012 Dec 27;7(12):e52354. doi: 10.1371/journal.pone.0052354

Figure 7. Western blot for nuclear factor-kappa B (NF-κB) activation.

Figure 7

A. SH-SY5Ycells transfected with the Swedish-APP mutant (Sw-APP) had significantly higher level of nuclear NF-κB p65 than cells transfected with wild-type APP (APP), which had higher level of nuclear NF-κB p65 than cells transfected with empty-vector (vector). The control nuclear protein was histone 3. B Image J and statistical analysis confirmed statistically significant difference in the level of NF-κB activation. Sw-APP transfected SH-SY5Ycells under greatest Aβ neurotoxicity had the highest level of nuclear NF-κB p65 more than that of wt-APP transfected cells (p<0.0001), which had higher level of NF-κB p65 than empty-vector transfected cells without Aβ neurotoxicity (p<0.001). Results shown were mean values from 3 independent cultures, and compared by one-way ANOVA using Newman-Keuls multiple comparison test.