Table 3. RAD51 protein multiple sequence alignment characterization.
| Sequencesource | Sequence length | Ave. number of substitutions per position | SIFT: median sequence conservation score | Percent amino acid sequence identity in pairwise comparison | ||||||||||||||
| Hsap | Mmus | Rnor | Ocun | Cfam | Btau | Sscr | Mdom | Ggal | Xlae | Drer | Bflo | Cint | Spur | Dmel | ||||
| Hsap | 339 | na | na | 1.00 | ||||||||||||||
| Mmus | 339 | nm | 4.32 | 0.99 | 1.00 | |||||||||||||
| Rnor | 339 | nm | 4.32 | 0.99 | 0.99 | 1.00 | ||||||||||||
| Ocun | 339 | nm | 4.32 | 0.99 | 0.99 | 0.99 | 1.00 | |||||||||||
| Cfam | 339 | nm | 4.32 | 0.99 | 0.99 | 0.99 | 0.99 | 1.00 | ||||||||||
| Btau | 339 | nm | 4.32 | 0.99 | 0.99 | 0.99 | 0.99 | 1.00 | 1.00 | |||||||||
| Sscr | 339 | nm | 4.32 | 0.99 | 0.98 | 0.98 | 0.98 | 0.99 | 0.99 | 1.00 | ||||||||
| Mdom | 339 | nm | 4.32 | 0.96 | 0.97 | 0.97 | 0.96 | 0.96 | 0.96 | 0.95 | 1.00 | |||||||
| Ggal | 339 | nm | 4.32 | 0.96 | 0.96 | 0.96 | 0.95 | 0.95 | 0.96 | 0.94 | 0.95 | 1.00 | ||||||
| Xlae | 336 | nm | 4.32 | 0.96 | 0.95 | 0.95 | 0.94 | 0.95 | 0.95 | 0.95 | 0.93 | 0.94 | 1.00 | |||||
| Drer | 340 | nm | 4.32 | 0.90 | 0.90 | 0.89 | 0.90 | 0.90 | 0.90 | 0.90 | 0.88 | 0.88 | 0.91 | 1.00 | ||||
| Bflo | 339 | nm | 4.32 | 0.85 | 0.84 | 0.85 | 0.84 | 0.84 | 0.85 | 0.84 | 0.83 | 0.83 | 0.85 | 0.83 | 1.00 | |||
| Cint | 338 | 0.64 | 4.32 | 0.82 | 0.82 | 0.82 | 0.81 | 0.82 | 0.82 | 0.82 | 0.82 | 0.80 | 0.80 | 0.81 | 0.78 | 1.00 | ||
| Spur | 335 | 0.80 | 4.32 | 0.84 | 0.84 | 0.84 | 0.83 | 0.84 | 0.84 | 0.84 | 0.84 | 0.84 | 0.84 | 0.83 | 0.82 | 0.80 | 1.00 | |
| Dmel | 336 | 1.19 | 4.32 | 0.66 | 0.66 | 0.66 | 0.66 | 0.66 | 0.66 | 0.66 | 0.65 | 0.66 | 0.67 | 0.68 | 0.64 | 0.64 | 0.69 | 1.00 |
na Not applicable.
nm Not measured. These measurements were conducted by sequentially removing sequences from the “complete” alignment and then performing the analysis indicated. Thus there was no need to continue after the alignment failed to meet either informativeness criterion.
Species abbreviations: Hsap, Homo sapiens (human); Mmus, Mus musculus (mouse); Rnor, Rattus norvegicus (rat); Ocun, Oryctolagus cuniculus (rabbit); Cfam, Canis familiaris (dog); Btau, Bos taurus (cow); Sscr, Sus scofa (pig); Mdom, Monodelphis domesticus (grey, short tailed opossum); Monodelphis domestica (opossum); Ggal, Gallus gallus (chicken); Xlae, Xenopus laevis (frog); Drer, Danio rerio (zebrafish); Bflo, Branchiostoma floridae (lancelet); Cint, Ciona intestinalis (tunicate); Spur, Strongylocentrotus purpuratus (purple sea urchin); Dmel, Drosophila melanogaster (fruitfly).