Table 2. Timing of syngamy, 1st, 2nd, 3rd cleavage stages, cavity formation and initiation of hatching for embryos derived from the sperm of normal and obese males.
Developmental event | Normal | Obese | P value | |
Cleavage (hours post-fertilisation) | ||||
Syngamy | 12.63±0.09 | 12.86±0.07 | <0.05 | |
1st cleavage | 2-cell | 14.35±0.08 | 14.66±0.07 | <0.001 |
2nd cleavage | 3-cell | 35.51±0.14 | 36.35±0.15 | <0.0001 |
4-cell | 36.45±0.16 | 37.34±0.17 | <0.001 | |
3rd cleavage | 5-cell | 45.99±0.17 | 46.92±0.19 | <0.001 |
6-cell | 46.60±0.18 | 47.49±0.19 | <0.01 | |
7-cell | 47.33±0.19 | 48.16±0.20 | <0.01 | |
8-cell | 47.96±0.20 | 48.80±0.23 | <0.05 | |
Cavitation | 76.47±0.44 | 77.24±0.43 | NS | |
Hatching | 90.88±0.76 | 91.02±0.85 | NS |
Data are expressed as mean ± SEM. n = 200 embryos each per paternal group; NS = not significant.