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. 2012 Dec 27;8(12):e1003069. doi: 10.1371/journal.ppat.1003069

Figure 4. Differential activation profiles of MAP kinases in response to heat shock.

Figure 4

(A) Phosphorylation of C. albicans Mkc1, Cek1 and Hog1 during a 30°C–42°C heat shock revealed by western analysis using phospho-specific antibodies. Total kinase levels were monitored using antibodies against total Hog1, FLAG-tagged Mkc1 and TAP-tagged Cek1. Actin served as an internal loading control. (B) Expression of target genes for Mkc1 (PGA13), Cek1 (PMT4) and Hog1 (RHR2) during a 30°C–42°C heat shock, as determined by qRT-PCR of the corresponding transcripts relative to the internal ACT1 mRNA control.