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. 2004 Mar;48(3):1028–1031. doi: 10.1128/AAC.48.3.1028-1031.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 1. — Model of the site-specific deletion of the SRL PAI YSH6000. The integrated SRL PAI is deleted via site-specific recombination to form a reconstituted attB site and an extrachromosomal circular form of the SRL PAI. Thin lines represent chromosomal DNA, and a thick line represents the SRL PAI. The SRL is represented by a filled box, and attL (attBOP′), attR (attPOB′), attP (attPOP′), and attB (attBOB′) sites are represented as open boxes. Integrase (int) and serX genes are represented as large arrows, and the position and orientation of primers used in amplifications are indicated with small arrows. The diagram is not drawn to scale.