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. 2012 Dec 27;8(12):e1003077. doi: 10.1371/journal.ppat.1003077

Figure 6. Gene expression in PPDB-stimulated PBMC from BCG vaccinated and control cattle.

Figure 6

A. Protective efficacy after M. bovis challenge determined by pathology scoring. Results are expressed as total pathology scores [5]. Filled circles, unvaccinated control calves; open triangles, BCG vaccinated calves. (B, C). Transcription of the genes expressing IFN-γ (B) and IL-22 (C) following in vitro stimulation. PBMC were collected from BCG vaccinated (filled symbols) and controls (open symbols) before challenge (week -1) and after challenge with M. bovis at weeks 2, 4 and 8, and stimulated with PPD-B for 24 hours. cDNA was prepared and gene expression determined by RT-qPCR. Data are expressed as log10 relative expression levels compared to non-stimulated cells. Statistical analysis: 2-way ANOVA with Bonferroni post test, * P<0.05.