Skip to main content
. 2012 Dec 27;6(12):e1968. doi: 10.1371/journal.pntd.0001968

Figure 1. Coinjection of plasmids encoding IL-4 and MIP-1α+FLT3L marginally improves immunisation with LsALT-expressing plasmids.

Figure 1

Mice were immunised with DNA plasmids expressing parasite LsALT (ALT) and murine IL-4 (pIL4) or MIP-1α (pMIP) and Flt3L (pFlt3L). Empty expression plasmid (pEmpty) was injected as a control for DNA-induced inflammation to equalise DNA quantities between groups. All groups were subsequently challenged with live infective L. sigmodontis larvae subcutaneously and infection was allowed to progress for 10 days. (A–B), LsALT-specific IgG1 and IgG2a, respectively and (C), concentrations of total IgE in the serum were increased by the coadministration of pMIP and pFlt3L compared to ALT alone (b, P = 0.03). (D), Eosinophil recruitment to the site of infection, the pleural cavity. Cell enumerations were performed on pleural lavage fluid. (E), Parasite survival represented as the proportion of worms found in the pleural cavity of infected mice relative to the infective dose. Points represent individual mice at D10 p.i. (N = 5–6 mice per group).