Skip to main content
. 2012 Dec 27;6(12):e1968. doi: 10.1371/journal.pntd.0001968

Figure 4. Removal of asparaginyl endopeptidase inhibition by LsCPI increases its immunogenicity.

Figure 4

Mice were immunised against LsCPI with plasmids encoding either the native protein (CPI) or a plasmid expressing its mutated form (CPIm), in combination with pIL4+pFlt3L+pMIP or alone. All mice other than naïve were challenged with live infective L. sigmodontis larvae. (A), LsCPI-specific IgG1 (b vs. [a, ab], P = 0.02, 0.3), and (B), total serum IgE concentrations (a, P≤0.001). ELISAs were performed with recombinant LsCPI. (C), Parasite survival 60d after infection. pEmpty, non-coding plasmid control; CPI, pcDNA 3.1 plasmid with an insert encoding LsCPI; CPIm, mutated form of LsCPI; adj = pIL4+pFlt3L+pMIP. Points represent individual mice at D60 p.i. (N = 5 mice per group).