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. 2012 Dec 27;6(12):e1989. doi: 10.1371/journal.pntd.0001989

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© 2012 Mousson et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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At 14 days post-DENV-2 infection, salivary glands were dissected, fixed, and then incubated simultaneously with two Wolbachia probes and two DENV specific probes. In panels A, B, and C, DENV-2 (green) is labeled with FITC. In panels D, E, and F, Wolbachia (red) is stained with Rhodamine. In panels G, H, and I, the red and green channels are merged. A co-localization of Wolbachia and DENV-2 was detected in some cells (panel H). DENV-uninfected and Wolbachia-uninfected controls are presented in panel C and F, respectively. Scale bars: 50 µm.