Figure 6. Type I IFN and not p65 are important for lymphocyte recruitment.
Mice were inoculated with intranasal RV1B or PBS and harvested at various time points.
- P65+/− mice had similar BAL lymphocytes at 8–96 h compared to p65+/+ mice (n = 2–3 independent experiments with 4–6 mice per group or per time point per experiment), and had similar levels of BAL CCL5, CXCL10, and CXCL11 (n = 2 independent experiments with 4–6 mice per group or per time point per experiment). Data was analysed by two-way ANOVA, ns = not significant.
- IFNAR1−/− mice had reduced BAL lymphocytes from 48–72 h post infection compared to controls (wt, n = 2–3 independent experiments with 4–6 mice per group or per time point per experiment), and reduced BAL CCL5 at 24–48 h, reduced CXCL10 at 8 h elevated CXCL11 at 8 h which was reduced at 72 h (n = 2 independent experiments with 4–6 mice per group or per time point per experiment). Data was analysed by two-way ANOVA, ***p < 0.001, **p < 0.01 as indicated, all data are expressed as mean ± SEM.