Figure 2.

The active β1 integrin is internalized more efficiently than the inactive conformation. A) NCI-H 460 cells were grown under regular cell culture conditions. Cells were lifted on ice and stained for 1 h with the indicated monoclonal anti-β1 integrin antibodies. Cells were lifted back to +37°C and allowed to endocytose the primary antibodies for 30 min. Cells were fixed, permeabilized and stained with secondary antibodies. The mid-section of a confocal stack is shown. B) The intensity of endocytosed integrin was quantified from the confocal images (n = 10–16) using a region of interest (ROI) inside the cell and normalized against the total staining of the cell. Scale bar 10 µm. Columns show mean + standard error of the mean. p V alues are calculated using M ann– W hitney test.