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. 2012 Jan 31;13(4):610–625. doi: 10.1111/j.1600-0854.2012.01327.x

Figure 3.

Figure 3

Antibody- and quenching-based method allows direct measurement of integrin trafficking. A) Outline of the antibody-based integrin trafficking assay for measuring endocytosis and recycling. B) Comparison of β1 integrin endocytosis in MDA-MB -231 cells with antibody-based and biotin– IP -based assays using total β1 integrin K 20 antibody. Graph shows mean ± standard error of the mean ( SEM ) of three independent experiments. Total surface and endocytosed biotin-labelled β1 integrin are shown in the western blot. C) Comparison of β1 integrin recycling in MDA-MB -231 cells with antibody-based and biotin– IP -based assays using total β1 integrin K 20 antibody. PQ was used to block the endosomal recycling of integrins (green line). Graph shows mean ± SEM of three independent experiments. Total surface, total endocytosed (30 min) and recycled biotin-labelled β1 integrins are shown in the western blot.