Table 1.
Efficacy of the peptides in disrupting the PP1:I2 complex.[a]
| Peptide | Sequence | EC50 [nm] |
|---|---|---|
| PDP0 | RVTFSEDDEII | 87±10 |
| PDP1 | RVTFAEAAEII | 21±2 |
| PDP2 | RVTFAEAAEII | 53±8 |
| PDP2m | RATAAEAAEII | inactive |
| PDP3 | RVTFBpaEAAEII | 176±13 |
| PDP3m | RATABpaEAAEII | inactive |
[a] See also Figure S1 in the Supporting Information. The results are presented as the mean±standard error of the mean (n=4). The efficacy of the peptides was determined by an in vitro phosphatase assay, measuring the activity of PP1 toward its 32P-labeled substrate glycogen phosphorylase a.[9]