. 2012 Dec 21;18(47):7093–7099. doi: 10.3748/wjg.v18.i47.7093

Table 1.

Primer sequences, polymerase chain reaction and denaturing high-performance liquid chromatography conditions for detection of gene polymorphisms

Gene	Primer sequence	PCR annealing temperature (°C)	PCR product size (bp)	DHPLC application type	Oven temperature (°C)
IL-1B-31	F: AGAAGCTTCCACCAATACTC	60	240	Mutation	59
IL-1B-31	R: AGCACCTAGTTGTAAGGAAG	60	240	Mutation	59
IL-1B-511	F: TGGCATTGATCTGGTTCATC	58.5	306	Mutation	60.5
IL-1B-511	R: GTTTAGGAATCTTCCCACTT	58.5	306	Mutation	60.5
IL-1 RN	F: CCCCTCGAGCAACATCC	59	270-442	VNTR	50.0
IL-1 RN	R: GGTCAGAAGGGCAGAGA	59	270-442	VNTR	50.0

DHPLC: Denaturing high-performance liquid chromatography; VNTR: Variable number of identical tandem repeats; PCR: Polymerase chain reaction; IL: Interleukin.