Table II.
Neutralization of HIV by Milk Derived b12 IgA2a
| CHO Purifiedb |
Milk Purified | Milk Expressed | |
|---|---|---|---|
| SF162 (IC90)c | 1.03 ± 0.52 | 0.79 ± 0.29 | 0.34 ± 0.13d |
| 67970 (IC50)c | 8.68 ± 3.05 | 12.94 ± 5.47 | 1.52 ± 0.73d |
| JR-FL (IC50)c | 7.27 ± 2.45 | 27.60 ± 16.17 | 2.72 ± 1.49d |
| 89.6 (IC90) c | 6.75 ± 2.63 | 8.37 ± 3.10 | 1.85 ± 1.53d |
a
Neutralization was measured using TZM-bl assay
b
b12 IgA2 antibody was purified from CHO cells, milk and tested as expressed in milk.
c
Virus isolate tested with indication of either IC90 or IC50 values were used as determined by linear regression analysis
d
Enhanced neutralization by milk-expressed b12 IgA2 reached statistical significance when compared to purified antibody (CHO or milk) with p<0.05