TABLE 1.
Primer sequences and amplicon sizes (base pairs, bp) of cDNAs coding for neurotransmitter receptors, nitric oxide-related enzymes, neural markers, transcription factors, and GAPDH used for real-time PCR
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | bp | Ref. |
|---|---|---|---|---|
| P2X1 | GAGAGTCGGGCCAGGACTTC | GCGAATCCCAAACACCTTGA | 233 | 66 |
| P2X2 | TCCCTCCCCCACCTAGTCAC | CACCACCTGCTCAGTCAGAGC | 149 | 66 |
| P2X3 | CTGCCTAACCTCACCGACAAG | AATACCCAGAACGCCACCC | 150 | 66 |
| P2X4 | CCCTTTGCCTGCCCAGATAT | CCGTACGCCTTGGTGAGTGT | 145 | 66 |
| P2X5 | GGATGCCAATGTTGAGGTTGA | TCCTGACGAACCCTCTCCAGT | 81 | 66 |
| P2X6 | CCCAGAGCATCCTTCTGTTCC | GGCACCAGCTCCAGATCTCA | 152 | 66 |
| P2X7 | GCACGAATTATGGCACCGTC | CCCCACCCTCTGTGACATTCT | 171 | 66 |
| P2Y1 | AACCGTGATGTGACCACTGA | TTCAACTTGTCCGTTCCACA | 216 | 67 |
| P2Y2 | TGCTGGGTCTGCTTTTTGCT | ATCGGAAGGAGTAATAGA | 209 | 67 |
| P2Y4 | TCGATTTGCAAGCCTTCTCT | CCATAGGAGACCAGGGTGAT | 215 | 67 |
| P2Y6 | TGCTGCTACCCCCAGTTTAC | TGGCATAGAAGAGGAAGCGT | 246 | 67 |
| P2Y12 | CTGTTTTTTGCTGGGCTCATC | GCGGATCTGGAAGAAAATCCT | 60 | |
| P2Y13 | GGATGCAGGGCTTCAACAA | GCAGCTGTGTCATCCGAGTGT | 60 | |
| P2Y14 | GGTGGGTTTCGCCTCATGT | CCTCAGGTGACCGGCATCT | 56 | |
| M1 mAChR | CTGTCACGGTCATGTGTACACTGT | CCGGGCTCGGTTTTCTGT | 62 | |
| M2 mAChR | CAAAAATGGCAGGCATGATG | GGCCCAGAGGATGAAGGAAA | 59 | |
| M3 mAChR | CGACGTGGTGTGATGATTGG | ATGGCAGGAGCCCATAGGA | 62 | |
| M4 mAChR | CACCAAACCCCTCACCTATCC | CGATCATGAGACCTGCCATCT | 59 | |
| M5 mAChR | CCATAATCCTGTCCGAATGGA | ATCCCGTGGCATTAGTTAGCA | 64 | |
| eNOS | GACTTTTAAGGAAGTAGCCAATGCA | CCATACAGGATAAGTCGCCTTCAC | 93 | |
| nNOS | CCAATGTTCACAAAAAACGAGTCT | TCGGCTGGACTTAGGGCTTT | 77 | |
| Argininosuccinate synthetase | TGCACTCTATGAGGACCGCTATC | AGGCCTGGCGAGAGAGGTGCCTAG | 49 | |
| B1BkR | CCAGGGTTCGTCATCACTATCTG | GCAAAAGGAAGAAGGACAAGACTAA | 73 | 68 |
| B2BkR | CCCTTCCTCTGGGTCCTCTT | CAGAACACGCTGAGGACAAAGA | 65 | 68 |
| β3-Tubulin | GAGACCTACTGCATCGACAATGAAG | GCTCATGGTAGCAGACACAAGG | 111 | 25 |
| GFAP | AAGAGTGGTATCGGTCCAAGTTTG | CAGTTGGCGGCGATAGTCAT | 107 | 25 |
| S100β | TGGTTGCCCTCATTGATGTCT | CCCATCCCCATCTTCGTCC | 179 | |
| GAPDH | TGCACCACCAACTGCTTAG | GGATGCAGGGATGATGTTC | 117 | 66 |
| Ngn1 | CAGTAGTCCCTCGGCTTCAG | AAGCAGGGTGTCGTATGGAG | 102 | 69 |
| Notch1 | CACCACGCCTCTCCCACCTGCCTGTAGC | TGCCTGTGTGCTTAGTGTGCCCGGAGTC | 209 | 70 |
| Stat3 | TATCTTGGCCCTTTGGAATG | GTGGGGATACCAGGATGTTG | 284 | 71 |
| NeuroD1 | CTTCCCGGTGCATCCCTACTCCTACC | AGGAAGGGCTGGTGCAATCAGTTAGG | 167 | 70 |