FIGURE 7.

Metformin stimulates CAP promoter in a JNK- and AMPK-dependent manner. A, promoter analysis of CAP in 3T3-L1 preadipocytes is shown. Cells were stimulated for 30 min with 10 mm metformin. Promoter activity was then measured. *, p < 0.05 versus CAP-Luc vector. B, cells were transiently transfected with AMPKα2 and JNK siRNA. After 48 h, cells were treated with 10 mm metformin for 30 min. Promoter activity was then measured. Each value is expressed as the mean ± S.E. (error bars) of three independent determinants. *, p < 0.05 versus metformin-treated in CAP vector transfection.