FIGURE 4.

NF-κB and MAPK activation induced by C. albicans hyphae and yeast are TAK1-dependent. A, flow cytometric analysis of BMDMs cultured with macrophage colony-stimulating factor (M-CSF) containing medium from LysM^Cre/+, TAK1^+/+LysM^cre/+TNFR1^−/−, and TAK1^F/−LysM^cre/+TNFR1^−/− mice with anti-F4/80, and anti-CD11b. B, LysM^Cre/+ (WT), TAK1^+/+LysM^cre/+TNFR1^−/− (TNFR1-KO), TAK1^F/−LysM^cre/+TNFR1^−/− (TNFR1-KO/TAK1-KO) BMDMs were either untreated or treated with C. albicans hyphae (MOI: 1) or heat-inactivated yeast (MOI: 5) for 90 min. The nuclear extracts were prepared from these cells and then subjected to the electrophoretic mobility shift assay using ³²P-labeled NF-κB or OCT-1 probe. C, LysM^Cre/+ (WT), TAK1^+/+LysM^cre/+TNFR1^−/− (TNFR1-KO), TAK1^F/−LysM^cre/+TNFR1^−/− (TNFR1-KO/TAK1-KO) BMDMs were stimulated with C. albicans hyphae for the indicated time points. Total cell lysates were prepared from these cells and then subjected to Western blotting. Unstim., unstimulated.