FIGURE 2.

Glycosyl composition and linkage analysis of lipoglycans from C. glutamicum and C. glutamicum ΔaftE. a, for glycosyl compositional analysis, purified lipoglycans were hydrolyzed, reduced, and per-O-acetylated as described under “Experimental Procedures.” Resulting alditol acetates were subjected for GC analysis. b, for determining the sugar linkages present in these lipoglycans species, lipoglycans were per-O-methylated, hydrolyzed, reduced, and per-O-acetylated. The resulting partially per-O-methylated, per-O-acetylated alditol acetates were analyzed using GC-MS. The partially per-O-methylated, per-O-acetylated alditol acetates were identified based on their characteristic alditol cleavage acetal profiles: t-Ara (m/z 118, 161), t-Man (m/z 118, 102, 145, 161, 162, 205), 2-Man (m/z 129, 130, 161, 190), 6-Man (m/z 118, 129, 162, 189, 233), and 2,6-Man (m/z 129, 130, 189, 190).