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. 2012 Nov 9;287(53):44173–44183. doi: 10.1074/jbc.M112.402396

FIGURE 2.

FIGURE 2.

Glycosyl composition and linkage analysis of lipoglycans from C. glutamicum and C. glutamicum ΔaftE. a, for glycosyl compositional analysis, purified lipoglycans were hydrolyzed, reduced, and per-O-acetylated as described under “Experimental Procedures.” Resulting alditol acetates were subjected for GC analysis. b, for determining the sugar linkages present in these lipoglycans species, lipoglycans were per-O-methylated, hydrolyzed, reduced, and per-O-acetylated. The resulting partially per-O-methylated, per-O-acetylated alditol acetates were analyzed using GC-MS. The partially per-O-methylated, per-O-acetylated alditol acetates were identified based on their characteristic alditol cleavage acetal profiles: t-Ara (m/z 118, 161), t-Man (m/z 118, 102, 145, 161, 162, 205), 2-Man (m/z 129, 130, 161, 190), 6-Man (m/z 118, 129, 162, 189, 233), and 2,6-Man (m/z 129, 130, 189, 190).