FIGURE 3.

hLM is a stronger inducer of Th17 cells than LAM. Naive CD4⁺ T cells from DO11.10 mice were co-cultured with BALB/c DC in the presence of 2.5 μm peptide OVA(323–339) and/or 10 μg of C. glutamicum LAM or hLM for 10 days. a, graphs and dot plots of FACS data of IL-17 and IFN-γ production by differentiated CD4⁺ T cells restimulated with PMA and ionomycin in the presence of brefeldin A. Each dot plot and column represents the mean of three wells. b, cytokine production by DC generated from BALB/c mice and stimulated for 24 h with LAM or hLM (10 μg/ml) or left unstimulated (Medium) was evaluated by ELISA. In all cases, results are from one representative of two independent experiments. Each column represents the mean ± S.E. (error bars) of three wells. Statistical significance was calculated with one-way ANOVA post hoc Tukey's t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001). bld, below detection level.