TABLE 3.
Intracellular levels of GSH and glutamate in S. cerevisiae strains grown in different media
| Strain | GSHa + additions to SCD |
Glutamateb + additions to SCD |
||||
|---|---|---|---|---|---|---|
| 1 mm Glutamate | 10 mm Glutamate | 10 mm GSH | 1 mm Glutamate | 10 mm Glutamate | 10 mm GSH | |
| nmol/mg protein | nmol/mg protein | |||||
| WT | 121.4 ± 8.6 | 131.5 ± 10.6 | 137.3 ± 15.1 | 11.1 ± 3.3 | 19.6 ± 3.8 | 13.2 ± 4.6 |
| Δgdh1 | 118.7 ± 10.5 | 123.7 ± 5.8 | 139.4 ± 7.9 | 9.9 ± 2.2 | 18.9 ± 6.7 | 14.4 ± 3.3 |
| Δgdh2 | 125.9 ± 3.4 | 133.7 ± 11.5 | 134.5 ± 19.3 | 13.4 ± 4.4 | 22.6 ± 5.5 | 15.7 ± 2.1 |
| Δgdh3 | 31.7 ± 10.6 | 120.5 ± 18.3 | 105.1 ± 32.1 | 2.1 ± 0.7 | 17.4 ± 4.2 | 10.1 ± 4.2 |
| Δgdh1Δgdh2 | 120.4 ± 5.8 | 125.7 ± 10.4 | 136.7 ± 4.7 | 11.5 ± 1.9 | 20.5 ± 0.8 | 16.2 ± 2.0 |
| Δgdh1Δgdh3 | 35.1 ± 6.0 | 115.6 ± 10.1 | 110.6 ± 10.6 | 1.9 ± 0.7 | 16.8 ± 5.2 | 10.8 ± 1.1 |
| Δgdh2Δgdh3 | 81.7 ± 19.1 | 124.6 ± 4.1 | 99.8 ± 4.6 | 6.1 ± 2.5 | 18.1 ± 3.8 | 12.5 ± 2.6 |
a Values were obtained from [(GSH + GSSG) − GSSG] and are presented as mean ± S.E. from three independent experiments. The percentage of GSSG to total cellular glutathione pool (GSH + GSSG) was <10% in all strains.
b Values are presented as mean ± S.E. from three independent experiments.