TABLE 4.

Intracellular levels of NADP-GDH and NAD-GDH activities in S. cerevisiae strains

Strain	NADP-GDH-specific activityα		NAD-GDH-specific activityα
	24 hb	48 hb	24 hb	48 hb
WT	1.224 ± 0.102	1.451 ± 0.126	0.042 ± 0.002	0.051 ± 0.003
Δgdh1	0.121 ± 0.006	1.316 ± 0.092	0.039 ± 0.001	0.057 ± 0.003
Δgdh2	1.213 ± 0.083	1.413 ± 0.117	<0.002	<0.002
Δgdh3	1.144 ± 0.088	0.159 ± 0.054	0.048 ± 0.002	0.049 ± 0.004
Δgdh1Δgdh2	0.120 ± 0.090	1.307 ± 0.125	<0.002	<0.002
Δgdh1Δgdh3	<0.005	<0.005	0.045 ± 0.003	0.055 ± 0.005
Δgdh2Δgdh3	1.219 ± 0.099	0.234 ± 0.016	<0.002	<0.002
Δgdh1Δgdh3/YCpGdh1	1.145 ± 0.108	0.187 ± 0.013	0.044 ± 0.001	0.054 ± 0.002
Δgdh1Δgdh3/YCpGdh3	0.122 ± 0.009	1.345 ± 0.117	0.041 ± 0.003	0.052 ± 0.003
Δgdh1Δgdh3/YCpPGDH1-Gdh3	1.253 ± 0.081	1.559 ± 0.089	0.047 ± 0.003	0.058 ± 0.004
Δgdh1Δgdh3/YCpPGDH3-Gdh1	0.007 ± 0.001	0.222 ± 0.014	0.046 ± 0.004	0.053 ± 0.005

^α Values given in μmol/min/mg protein and presented as mean ± S.E. from three independent experiments.

^b Length of time cells were cultured in SCD.