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. 2012 Nov 16;287(53):44234–44248. doi: 10.1074/jbc.M112.364109

FIGURE 1.

FIGURE 1.

The CB1 receptor modulates GPR55 transcription factor activation in HEK-GPR55+CB1 cells. HEK-GPR55, HEK-CB1, and HEK-GPR55+CB1 cells were transfected with NFAT (A, C, and E) or SRE (B, D, and F) transcription factor-luciferase-reporter plasmids. 24 h post-transfection, cells were stimulated with 1 μm LPI (A and B), 1 μm SR141716A (C and D), or with increasing concentrations of the selective GPR55 agonist GSK319197A (E and F) for 4 h in serum-free medium. NFAT activation (A, C, and E) and SRE induction (B, D, and F) was observed in HEK-GPR55 (white bars or ■), but not in HEK-CB1 (gray bars or ▴) cells. NFAT activation and SRE induction were reduced (A and B) or abolished (C–F) in HEK-GPR55+CB1 cells (black bars or ▾). Data are mean ± S.E. from one of four independent experiments performed in triplicates. Data were normalized and expressed as percent of maximum activation which was set as 100%, relative light units (RLU). *, p < 0.05; **, p < 0.01; ***, p < 0.001.