FIGURE 4.

The functional properties of BST-2 deletions do not correlate with the size of the deletions. A, schematic diagram of constructs used in this experiment. Transmembrane domains (TM1, TM2) and the position of the HA tag are indicated as black and shaded boxes, respectively. Dashed boxes indicate deletions. Numbers refer to amino acid positions. B, expression and dimerization of BST-2 variants was tested as described in the legend to Fig. 1B. C, 293T cells were transfected with 5 μg each of NL4–3/Udel together with varying amounts of BST-2 wt (closed circles) or mutant DNA (open circles) as indicated. Virus-containing supernatants were collected 24 h later and used to infect HeLa TZM-bl indicator cells. Analysis of the data were performed as described in the legend to Fig. 1C. The signal produced by NL4–3/Udel virus in the absence of BST-2 was each defined as 100% infectivity.