TABLE 2.
p53 variant | Tm (measured by DSF) | Tm (measured by DSC) | Rate of denaturation (relative to WT) | Kd (DNA)a |
---|---|---|---|---|
°C | °C | nm | ||
WT Dmp53 | 45.8 | 46.5 | Same | 180 ± 10 |
R155H | —b | —b | —b | NDc |
G233S | 41.8 | 41.8 | Fast | ND |
K235S | 42.2 | 43.8 | Fast | 310 ± 20 |
R268W | 45.2 | 45.1 | Same | ND |
K259H | Not measured | 46.4 | Same | ND |
QMHp53 | 51.2 | 52.1 | Slow | 150 ± 5 |
a Binding affinity to the gadd45 response element measured by fluorescence anisotropy at a total ionic strength of 225 mm.
b — indicates not detectable.
c ND = No detectable binding in μm range.