Skip to main content
. 2012 Nov 12;287(53):44338–44344. doi: 10.1074/jbc.M112.428987

FIGURE 2.

FIGURE 2.

PERKi blocks ER stress-inducible PERK kinase activity in vivo. A, immunoblot analysis of puromycin incorporation into total protein in untreated Min6 beta cells or cells exposed to thapsigargin (0.5 μm for 30 min) and the indicated concentrations of PERKi. Puromycin (10 μg/ml) was added during the last 10 min, immediately before harvest. B, quantification of the signal from three replicates of the experiment (shown in the inset) of which A is a typical representative. C, nonlinear regression analysis of the concentration-dependence of PERKi reversal of thapsigargin-mediated translational repression (n = 3, shown in A and B) and phosphorylation of eIF2α (n = 4, one of which is shown in A). D, immunoblot of total puromycinylated proteins, phosphorylated (eIF2αP), and total eIF2αT in wild-type (WT), EIF2aS51A, and Perk−/− MEFs exposed to PERKi (2 μm) and thapsigargin (0.5 μm) as indicated.