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β-Glucogallin assay in tea leaves by HPLC. For HPLC analysis, the solvent system consisted of 1% acetic acid in water (A) and 100% acetonitrile (B). After injection (5 μl), a linear gradient at a flow rate of 1.0 ml min−1 was established as follows: B from 0 to 10% (v/v) in 10 min was initiated, then B from 10 to 30% (v/v) between 10 min to 30 min. Peaks were identified by comparing the retention times with the standard. mAU, absorbance units.
