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Protein purification of ECGT enzyme. A, t-butyl hydrophobic interaction chromatography of ECGT enzyme is shown. B, affinity chromatography of ECGT enzyme on ConA-Sepharose is shown. C, gel chromatography of ECGT enzyme on Superdex 200 is shown. D, SDS-PAGE analysis of the ECGT purification steps of affinity and gel chromatography is shown. Lane M, protein markers; lane 1, ConA-Sepharose; lane 2–9, separations of peak in Superdex 200. AU, absorbance units.
