TABLE 1.

Purification of ECGT from tea plant

Purification step	Total protein(mg)	Total activity	Specific activity	Enzyme activity yield	Purification -fold
		pKat	pKat mg−1	%
Extraction	425.20	1703.96	4.01
20–60% ammonium sulfate fraction	221.11	1450.02	6.56	85.09	1.64
t-Butyl hydrophobic interaction chromatography	16.77	1106.10	65.94	64.91	16.45
Con A-Sepharose	0.14	431.73	3084.02	25.34	769.55
Superdex 200	0.05	306.76	6576.82	0.18	1641.09