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. 2012 Nov 14;287(53):44508–44517. doi: 10.1074/jbc.M112.424903

FIGURE 1.

FIGURE 1.

KDM6B is required for TGF-β-induced EMT. A, NMuMG cells were treated with TGF-β (10 ng/ml) for 24 h. B, Western blotting of EMT markers in the total protein extracted from control and TGF-β-treated NMuMG cells. C, real-time RT-PCR showing the induction of Kdm6b expression in response to TGF-β treatment in NMuMG cells. Values are mean ± S.D. (error bars) of triplicate samples from a representative experiment. D, NMuMG cells were infected with lentiviruses expressing Kdm6b shRNA or scramble shRNA. Kdm6b mRNA was determined by real-time RT-PCR. Values are mean of triplicate samples from a representative experiment. E, NMuMG/Scrsh and NMuMG/Kdm6bsh cells were treated with TGF-β for 24 h. F, NMuMG/Scrsh and NMuMG/Kdm6bsh cells were treated with TGF-β for different time points as indicated. G, NMuMG/Scrsh and NMuMG/Kdm6bsh cells were treated with TGF-β for 48 h, and then immunofluorescence staining was performed.