FIGURE 2.

KDM6B is required for TGF-β-induced EMT in hMLE cells. A, hMLE cells were treated with TGF-β for different times, and KDM6B expression was quantified by real-time RT-PCR. Values are mean ± S.D. (error bars) of triplicate samples from a representative experiment. B, MCF-10A cells were treated with TGF-β for the indicated times, and KDM6B mRNA was quantified by real-time RT-PCR. C, hMLE cells were infected with lentiviruses expressing scramble shRNA and KDM6B shRNA, and KDM6B mRNA was examined by real-time RT-PCR. D, hMLE/Scrsh and hMLE/KDM6Bsh1 and hMLE/KDM6Bsh2 cells were treated with TGF-β for 8 days. Total cell lysates were prepared and examined by Western blot analysis.