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. 2012 Nov 14;287(53):44508–44517. doi: 10.1074/jbc.M112.424903

FIGURE 3.

FIGURE 3.

The overexpression of KDM6B promotes EMT. A, MCF-10A cells were infected with retroviruses expressing KDM6B or empty vector. KDM6B mRNA was analyzed by real-time RT-PCR. B, the overexpression of KDM6B in MCF-10A cells induced an EMT-like phenotype. C, the total cell lysates were prepared from MCF-10A/EV and MCF-10A/KDM6B cells and subjected to Western blotting analysis. D, SNAI1 mRNA was examined by real-time RT-PCR. E, MDCK cells were infected with retroviruses expressing KDM6B or empty vector, and KDM6B mRNA was analyzed by real-time RT-PCR. F, the overexpression of KDM6B in MDCK cells induced spindle-shaped morphology and immunofluorescence staining showed the loss of E-cadherin expression with induction of N-cadherin expression. G, the whole cell extracts were isolated from MDCK/EV and MDCK/KDM6B cells and examined by Western blot. H, the expression of SNAI1 and SNAI2 was examined by real-time RT-PCR. Error bars, S.D.