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Quantifiable TG products (DP5 and DP6) accumulated by SpChiD and its mutants. Reaction mixtures containing 2 mm DP4 and 350 nm of each SpChiD and its indicated mutants were incubated separately for different time periods from 0 to 360 min at 40 °C. Products were quantified from respective peak areas by using standard calibration curves of CHOS. Comparative profiles show the quantity of DP5 and DP6 TG products produced at 30 min (A) and 6 h (B).
