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. 2012 Nov 7;287(53):44654–44666. doi: 10.1074/jbc.M112.404228

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Site-specific gold labels track nucleotide-dependent conformational changes. A, density corresponding to a gold cluster covalently attached to L5 (via T126C shown in a space-filling representation) is superimposed on the K5-MD reconstructions in rigor and AMPPNP and confirms the reorientation of L5 when AMPPNP binds. Gold difference maps in orange (rigor) and red (AMPPNP) are contoured at 2.6 and 2.4 σ, respectively. B, density corresponding to a gold cluster covalently attached to the NL (via V365C shown in a space-filling representation) is superimposed on the K5-MD reconstructions in rigor and AMPPNP and confirms the ordered-to-ordered transition of the NL toward the MT plus-end when AMPPNP binds. Gold difference maps in orange (rigor) and red (AMPPNP) are contoured at 1.6 and 2.4 σ, respectively.