Purification of MytiLec.
A, extract from mussel (M. galloprovincialis) by TBS containing 100 mm
d-Gal was extensively dialyzed and applied to a melibiose-conjugated agarose column (1 × 5 cm) equilibrated with TBS. MytiLec bound to the column was eluted with TBS containing 200 mm melibiose (arrow). B, SDS-PAGE pattern under reducing (R) and nonreducing (NR) conditions. Numbers at right indicate the molecular masses of marker proteins as follows: phosphorylase b (97 kDa), BSA (66 kDa), Ovalbumin (44 kDa), carbonic anhydrase (29 kDa), trypsin inhibitor (20 kDa), and lysozyme (14 kDa). M, molecular marker; C, crude extract; L, lectin.