FIGURE 3.

Antibodies binding to human milk SGM. The human milk SGM was interrogated with antibodies: anti-Le^a antibody (10 μg/ml; A), anti-SLe^a/LSTa antibody (10 μg/ml; B), anti-blood group H1 antibody (1:10 dilution; C), and anti-CD15 antibody (10 μg/ml; D). The microarray was also used to test the binding specificity of anti-TRA-1–60 antibody (50 μg/ml; E) and anti-TRA-1–81 antibody (50 μg/ml; F). Glycans 1–73 are sialylated glycans (tan), 74–127 are neutral glycans (violet), 128–140 are controls of structurally defined glycans (light green).