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TLR9 is specifically recruited to phagosomes containing Aspergillus fumigatus. Mouse macrophages expressing TLR9-GFP (green) and CD82-mRFP1 (red) were exposed to both live A. fumigatus (Af293) and fluorescent polystyrene beads (blue-5 μm). Twenty minutes after phagocytosis, macrophages had taken up both particles, but only phagosomes containing A. fumigatus recruited both CD82-mRFP1 and TLR9-GFP. Scale bar equals 5μm and arrow denotes internalized polystyrene bead.
